Fmoc-Gly-Wang Resin (100–200 Mesh): A Versatile Starting Point for Fmoc-SPPS
Fmoc-Gly-Wang Resin is a preloaded solid-phase synthesis resin, designed for Fmoc-based peptide synthesis, with glycine as the first amino acid already attached. Built on a polystyrene-divinylbenzene (PS-DVB) matrix and functionalized with a Wang linker, this resin is ideal for synthesizing peptides with a free carboxylic acid (-COOH) at the C-terminus.
The 100–200 mesh particle size offers an excellent compromise between mechanical stability and efficient reagent penetration, making it compatible with both manual and automated peptide synthesizers.
Product Specifications
Matrix: Polystyrene cross-linked with 1% divinylbenzene (DVB)
Functional Group: Wang linker preloaded with Fmoc-Glycine
Loading: Typically ~0.3–0.8 mmol/g (varies by supplier)
Particle Size: 100–200 mesh (~75–150 µm)
Swelling: Good swelling in DCM, DMF, and other aprotic solvents
Protecting Group: Fmoc (9-fluorenylmethoxycarbonyl), base-labile
Key Features
Preloaded with Fmoc-Gly: Eliminates the need for initial amino acid coupling, saving time and ensuring consistency.
Wang Linker: Acid-labile linker that releases peptides with a free C-terminal acid upon cleavage.
Fmoc Strategy Compatible: Fully suited to the widely used Fmoc/tBu peptide synthesis protocol.
High Purity and Consistency: Ensures reproducibility in peptide sequences and yields.
Applications
Peptide Synthesis: Ideal for synthesizing peptides starting with glycine at the N-terminus and terminating in a C-terminal free acid.
Structure-Activity Relationship (SAR) Studies: Streamlines the production of peptide analogs and libraries.
Bioconjugation Studies: Supports high-purity peptide synthesis for downstream labeling or conjugation.
Cleavage and Deprotection
Peptides can be cleaved from Fmoc-Gly-Wang resin using trifluoroacetic acid (TFA) in the presence of scavengers (e.g., water, TIS, EDT) to simultaneously remove protecting groups and release the free peptide.
Standard cleavage conditions: TFA cocktail (95% TFA, 2.5% water, 2.5% TIS) for 1–3 hours.
Storage and Handling
Store dry at room temperature, protected from light and moisture.
Swell in DCM, DMF, or other compatible solvents before use.
Avoid prolonged exposure to bases prior to synthesis to preserve the Fmoc group.
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